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Nucleic Acids Symposium Series 2001 1(1):11-12; doi:10.1093/nass/1.1.11
© 2001 by Oxford University Press
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Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides with the intercalators at the junction point

Yoshihito Ueno1,2, Mai Mikawa1, Shuichi Hoshika1, Masako Takeba1, Yukio Kitade2 and Akira Matsuda1

1 Graduate School of Pharmaceutical Sciences, Hokkaido University, 2 Department of Biomolecular Science, Faculty of Engineering, Gifu University

3'-3'-Linked oligodeoxynucleotides (ODNs) with the anthraquinonyl group at the junction point were synthesized on a DNA synthesizer using a controlled pore glass (CPG), which has pentaerythritol carrying the intercalator at one of the four hydroxymethyl groups. Stability of the triplexes with the target duplexes was studied by thermal denaturation. The 3'-3'-linked ODNs with the anthraquinonyl group enhanced the thermal stability of the triplexes when compared with those without the intercalator and the unmodified nonamer. The inhibitory activity of the 3'-3'-linked ODNs against the cleavage of the target DNA by the restriction enzyme Hind III was tested. It was found that the 3'-3'-linked ODN with the anthraquinonyl group at the junction point inhibited the cleavage by the enzyme more effectively than the nonamer and the 3'-3'-linked ODN without the intercalator.


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