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Nucleic Acids Symposium Series 2001 1(1):129-130; doi:10.1093/nass/1.1.129
© 2001 by Oxford University Press
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Synthesis and characterization of oligonucleotides containing formamidopyrimidine lesions (Fapy·dA, Fapy·dG) at defined sites

Kazuhiro Haraguchi, Michael O. Delaney, Carissa J. Wiederholt, Aruna Sambandam, Zsolt Hantosi and Marc M. Greenberg

Department of Chemistry, Colorado State University, Ft. Collins, CO 80523, USA

The preparation of oligonucleotides containing Fapy·dA (N4- (2-Deoxy-{alpha},ß-D-erythro-pentofuranosyl)-4,6-diamino-5-formamidopyrimidine) and Fapy·dG (N6-(2-Deoxy-{alpha},ß-D-erythro-pento-furanosyl)-2,6-diamino-4-hydroxy-5-formamido-pyrimidine) at defined sites was achieved by introducing the lesions as dinucleotide phosphoramidites. Oligonucleotides as composed of as many as 36-nucleotides were prepared by solid-phase synthesis and/or a combination of chemical synthesis and enzymatic ligation. Oligonucleotides containing non-hydrolyzable analogues were also prepared. Oligonucleotides containing these modified nucleotides were characterized by a variety of chemical and biochemical methods.


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