Skip Navigation

Nucleic Acids Symposium Series 2001 1(1):235-236; doi:10.1093/nass/1.1.235
© 2001 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Akashi, H.
Right arrow Articles by Taira, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akashi, H.
Right arrow Articles by Taira, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

A simple and rapid system for the quantitation of RNA interference in plant cultured cells

Hideo Akashi1, Makoto Miyagishi1, Hiroyuki Kurata2, Toshiyuki Nagata3 and Kazunari Taira1,4

1 Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan, 2 Department of Biochemical Engineering and Science, Kyushu Institute of Technology, 980-4 Kawazu, lizuka, Fukuoka 820-8502, Japan, 3 Department of Biology, Faculty of Science, The University of Tokyo, Hongo, Tokyo 113-8656, Japan, 4 Gene Discovery Research Center, National Insitute of Advanced Industrial Science and Technology (AIST), MITI, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan

The phenomenon known as RNA interference (RNAi) by double-stranded RNA (dsRNA) that was reported recently in the nematode Caenorhabditis elegans has been shown to operate by a mechanism that is widely conserved among species including plant cells. No quantitative analysis of the effects of RNAi on the expression of specific genes in plant cultured cells has been reported. An RNAi effect was observed 24 h after the introduction of dsRNA expression plasmids into tobacco BY-2 cells by electroporation. The simple system for suppression of specific genes in plant cells should be useful in attempts to elucidate the roles of individual genes in plant cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.