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Nucleic Acids Symposium Series 2001 1(1):25-26; doi:10.1093/nass/1.1.25
© 2001 by Oxford University Press
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The {alpha}-L-ribo-isomers of RNA and LNA (locked nucleic acid)

Mads D. Sørensen1, Lisbet Kværnø1, Torsten Bryld2, Anders E. Håkansson1, Lise Keinicke1, Birgit Verbeure3, Piet Herdewijn3 and Jesper Wengel2

1 Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark, 2 Department of Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark, 3 Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium

The phosphoramidite approach has been used for the automated synthesis of {alpha}-L-LNA, {alpha}-L-RNA, and oligomers composed of mixtures of {alpha}-L-LNA, {alpha}-L-RNA and DNA monomers. Binding studies revealed very efficient recognition of single-stranded DNA and RNA target oligonucleotide strands. {alpha}-L-LNAs were shown to be significantly stabilized towards 3'-exonucleolytic degradation. Duplexes formed between RNA and {alpha}-L-LNA induced E. coli RNase H-mediated RNA cleavage, albeit very slow, at high enzyme concentration.


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