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Nucleic Acids Symposium Series 2001 1(1):41-42; doi:10.1093/nass/1.1.41
© 2001 by Oxford University Press
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Evidence of G-A/A-G pair, not A-G/G-A pair, in SECIS element

Yuko Ito and Takaharu Mizutani

Graduate School of Pharmaceutical Science, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, Japan

The decoding of selenocysteine (Sec) codon for selenoprotein translation in animals requires the SECIS (selenocysteine insertion sequence) element in the 3'-untranslated region (3'-UTR) of selenoprotein mRNA. It has been hypothesized that SECIS has G-A/A-G pairs, which are non-Watson-Crick base pairs, and are one of the most indispensable parts for Sec translation. However, DNA structure research has shown that the DNA loop structure was closed by a G-A pair (1), although there is little evidence for G-A stability in SECIS. Our aim was to investigate the existence of a G-A/A-G base pair by electrophoretic mobility and Tm measurement, and we found that G-A/A-G bases formed a stable pair, but A-G/G-A bases did not.


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