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Nucleic Acids Symposium Series 2001 1(1):55-56; doi:10.1093/nass/1.1.55
© 2001 by Oxford University Press
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Photo-regulation of RNA polymerase reaction by use of modified DNA carrying an azobenzene

Hiroyuki Asanuma1, Daisuke Tamaru1, Takayuki Yoshida1, Akira Yamazawa1, Tsuneo Mitsui2, Taeko Okuni2, Michiko Kimoto2, Ichiro Hirao2, Shigeyuki Yokoyama2 and Makoto Komiyama1

1 Research Center for Advanced Science and Technology, The Univ. of Tokyo, Komaba, Meguro-ku, Tokyo 153-8904, Japan, 2 Yokoyama CytoLogic Project, ERATO, JST, c/o RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan

Transcription reaction by T7-RNA polymerase was photo-regulated on the basis of two strategies as depicted in Scheme 1. It was found that incorporation reaction of azobenzene-tethered uridine triphosphate proceeded only when azobenzene took trans-form (Scheme 1(A)). On the other hand, the transcription was more efficient when the azobenzene moiety, tethered to the non-template strand of the promoter DNA, was in its cis-form under UV irradiation (Scheme 1(B)). Thus, azobenzene-tethered DNAs are promising for the photo-regulation of gene-expression.


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