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Nucleic Acids Symposium Series 2001 1(1):61-62; doi:10.1093/nass/1.1.61
© 2001 by Oxford University Press
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Preparations of a RNA-lipid complex film and its physical properties

Yu Hoshino, Hajime Nakayama and Yoshio Okahata

Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, Japan

We extracted RNA (aver. 70–80 bases) from yeasts and prepared a RNA-lipid complex by replacing sodium counter cations with cationic amphiphiles (2C12N+2C1). The RNA-lipid complex was soluble only in organic solvents and formed a self-standing film by casting from organic solutions. The RNA film was physically strong, although its molecular weight is small and the RNA molecules are oriented in one direction by stretching the film, in which RNAs form intermolecular hydrogen bonds and show the physical strength of the film.


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