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Nucleic Acids Symposium Series 2003 3(1):43-44; doi:10.1093/nass/3.1.43
© 2003 by Oxford University Press
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Development of a novel genosensor based on ferrocenyl oligonucleotides

Kosuke Mukumoto1, Takahiko Nojima1, Nobuaki Furuno2 and Shigeori Takenaka1

1 Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan, 2 Institute for Amphibian Biology, Hiroshima University, Higashi-hiroshima 739-8526, Japan

Two types of ferrocenyl oligonucleotides, Fc1-ODN and Fc2-ODN, carrying a 5'-ATT GCT CAG GGG TAA GGT CAT TAG TTG GAA-3' sequence were allowed to hybridize with the DNA probe carrying a complementary sequence immobilized on the gold electrode to give rise to a redox signal deriving from their ferrocenyl moieties. When a mixture of these oligonucleotides in a proper ratio was used as a model of DNA samples, two redox peaks were observed in one differential pulse voltammogram. The peak currents vaned depending on the initial ratio of the two oligonucleotides, suggesting a possibility that this technique may be applied to electrochemical monitoring of gene expression by, for example, the electrochemical differential hybridization, EDH.


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