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Nucleic Acids Symposium Series 2000 44(1):11-12; doi:10.1093/nass/44.1.11
© 2000 by Oxford University Press
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Enzymatic properties of Escherichia coli and human 7,8-dihydro-8-oxoguanine DNA glycosylases

Kenjiro Asagoshi, Takao Yamada, Hiroaki Terato, Yoshihiko Ohyama and Hiroshi Ide

Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan

Oxidative damage to DNA generates aberrant guanine bases such as 2,6-diamino-4-hydroxy-formamido-pyrimidine (Fapy) and 7,8-dihydro-8-oxoguanine (8-oxoG). Although synthetic oligonucleotides containing a single 8-oxoG have been widely used to study enzymatic processing of this lesion, the synthesis of oligonucleotides containing Fapy as a unique lesion has not been achieved to date. In this study, an oligonucleotide containing a single 2,6-diamino-4-hydroxy-5-(N-methyl)formamido-pyrimidine (me-Fapy, a methylated derivative of Fapy) was prepared by a DNA polymerase reaction and the subsequent alkali treatment. The repair activity of Fpg and hOGGl proteins were compared using oligonucleotide substrates containing me-Fapy and 8-oxoG.


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