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Nucleic Acids Symposium Series 2000 44(1):3-4; doi:10.1093/nass/44.1.3
© 2000 by Oxford University Press
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Mutations induced by glyoxal and methylglyoxal in mammalian cells

Naoko Murata-Kamiya1, Hiroyuki Kamiya1,2, Hiroshi Kaji1 and Hiroshi Kasai1

1 Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan, 2 Present address: Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan

To investigate the mutation spectra of glyoxal and methylglyoxal in mammalian cells, we analyzed mutations in a bacterial suppressor tRNA (supF) gene in the shuttle vector plasmid pMY189. The cytotoxicity and the mutation frequency increased according to the doses of glyoxal and methylglyoxal. The majority of glyoxal-induced mutations (65%) were base-pair substitutions, in which G:C->C:G transversions were predominant. In the mutants induced by methylglyoxal, multi-base deletions were predominant (50%), followed by base-pair substitutions (35%), in which G:C->C:G and G:C->T:A transversions were predominant.


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