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Nucleic Acids Symposium Series 2000 44(1):47-48; doi:10.1093/nass/44.1.47
© 2000 by Oxford University Press
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Synthesis and properties of novel acyclic nucleotides

Masakazu Sakata1 and Masayuki Fujii1,2

1 Department of Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanorriori, lizuka, Fukuoka 820-8555, Japan, 2 Henkel Research Center of Advanced Technology, Molecular Engineering Institute, Kinki University, 11-6 Kayanomori, lizuka, Fukuoka 820-8555, Japan

Acyclic adenosine and thymidine analogs derived from L- and D-threonie were synthesised and incorporated into oligonucleotides by automated protocols using a standard phosphoramidite method. UV melting experiments with thus obtained oligonucleotides showed that incorporation of those acyclic nucleosides did not destabilize the hybrid duplexes and that the stabilities of them are ifluenced by the stereochemical structures of acyclic analogs. Modification of 3'-end of oligonucleotide with acyclic analogs protected the oligonucleotide against 3'-exonuclease.


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