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Nucleic Acids Symposium Series 2000 44(1):5-6; doi:10.1093/nass/44.1.5
© 2000 by Oxford University Press
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Analysis of histidine-dependent antitermination in Bacillus subtilis hut operon

Masanao Oda1, Noboru Kobayashi2, Yasurou Kurusu2 and Masaya Fujita3

1 National Institute of Bioscience and Human Technology, AIST, MITI, Tsukuba Science City, Ibaraki 305-8566, Japan, 2 Faculty of Agriculture, Ibaraki University, Ami, Ibaraki 300-03, Japan, 3 National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan

We have previously shown that a positive regulator,HutP, of Bacillus subtilis hut operon is a RNA binding protein. Here, we report precise binding site of HutP in cis-regulatory region on hut mRNA and the role of HutP in histidine-dependent antitermination of hut expression. Ethylnitrosourea modification interference assay showed that four binding sites of HutP were found in the cis-regulatory sequences and were located at the stem and the internal loop of an antiterminator structure. In vitro transcription assay indicated that HutP suppressed transcription termination in the presence of histidine. These results suggest that HutP function as an antiterminator in response to the presence of histidine.


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