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Nucleic Acids Symposium Series 2000 44(1):73-74; doi:10.1093/nass/44.1.73
© 2000 by Oxford University Press
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DNA lesion and mutagenesis induced in phageM13mp2 by UVA, UVB and UVC irradiation

Yuji Izumizawa1,2, Song-Ju Yang1,2, Tomoe Negishi1 and Kazuo Negishi2

1 Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan, 2 Gene Research Center, Okayama University, Tsushima, Okayama 700-8530, Japan

Sunlight is carcinogenic and mutagenic and its genotoxic effects are believed to be the result of UV light-induced lesions in DNA. These lesions include pyiimidine dimers and (6-4) photoproducts, but it is uncertain whether the pyrimidine modifications are the sole pre-mutagenic lesions induced by UV light. Previous studies indicate that some sunlight-induced mutations in the single-stranded DNA phage M13mp2 may not be caused by these photoproducts. In this work, purified single-stranded phage DNA was exposed to UVA, UVB and UVC and the induced mutations were analyzed. All 3 types of UV light increase the mutation frequency. The mutants were sequenced and the results suggest that UVA exposure may induce formation of a non-dipyrimidine lesion in DNA.


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