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Nucleic Acids Symposium Series 2000 44(1):9-10; doi:10.1093/nass/44.1.9
© 2000 by Oxford University Press
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Construction of an efficient expression vector for coupled transcription/translation in a wheat germ cell-free system

Tatsuya Sawasaki, Yoshinori Hasegawa, Masateru Tsuchimochi, Yuko Kasahara and Yaeta Endo

Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama 790-8577, Japan

Using the expression vector, pEU, which we have constructed, highly efficient in vitro protein synthesis can be achieved: The system works for 150 hours and without further template addition once the reaction has started, yielding 5 mg of enzymatically active protein in a 1 ml reaction.


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