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Nucleic Acids Symposium Series 2004 48(1):13-14; doi:10.1093/nass/48.1.13
© 2004 by Oxford University Press
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Synthesis and properties of 2'-O-cyanoethylated RNA derivatives

Hisao Saneyoshi1,3, Keigo Tamaki1,3, Kohji Seio2,3 and Mitsuo Sekine1,3

1 Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan, 2 Frontier Collaborative Research, Tokyo Institute of Technology, Japan, 3 CREST, JST (Japan Science and Technology Agency), Nagatsuda, Midoriku, Yokohama 226-8501, Japan

We developed a new protocol for the cyanoethylation of the hydroxyl function of nucleosides. A new system of Cs2CO3-tBuOH proved to be the best for the cyanoethylation. An oligonucleotide incorporating 2'-O-cyanoethylated uridine has high affinity ({Delta}Tm = 0.6 °C/modification) for the complementary DNA or RNA strand than the corresponding 2'-O-methyl-RNA.


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