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Nucleic Acids Symposium Series 2004 48(1):21-22; doi:10.1093/nass/48.1.21
© 2004 by Oxford University Press
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Synthesis and application of new amino modification analogues for functional oligonucleotide

Yasuo Komatsu1, Naoshi Kojima1, Ken Nonaka2, Yumi Fujinawa2, Maiko Sugino1, Akiko Mikami1, Jyunya Hashida3, Eiko Ohtsuka1 and Kenichi Matsubara2

1 Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science & Technology, Toyohira-ku, Sapporo 062-8517, Japan, 2 DNA Chip Research Inc., Tsurumi-ku, Yokohama 230-0045, Japan, 3 Hitachi Software Engineering Co., Ltd., Tsurumi-ku, Yokohama 230-0045, Japan

We synthesized new analogues to functionalize oligonucleotides. These analogues included a primary amino group tethering to an aromatic ring, and we introduced them into the 5'-end of each oligonucleotide. The oligonucleotides with the aromatic amino group (OAA) were easily purified from impurities by using their hydrophobic property of the aromatic residue. These OAA probes reacted with activated ester groups more efficiently than the conventional probe, which was modified with 6-aminohexyl group. Furthermore, we applied these OAAs to oligonucleotide array probes. The OAA probes were efficiently immobilized on the array surface, and the hybridization intensity on these probes increased as compared with the conventional probes. These new probes can be a new nucleic acid tool for biological assay systems.


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