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Nucleic Acids Symposium Series 2004 48(1):23-24; doi:10.1093/nass/48.1.23
© 2004 by Oxford University Press
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Selective nitrosyl group transfer reaction to cytidine using oligonucleotides bearing S-nitrosothioguanosine

Fumi Nagatsugi, Shizuka Nakayama, Monsur Md. Ali and Shigeki Sasaki

Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan, CREST, JST

Nitric oxide (NO) is an important endogenous regulatory molecule, and conversely, it is also known to be mutagenic because of its ability of nitrosylation and following deamination of nucleobases. In this study, we aimed at developing a useful tool for nitrosylation to a specific site of DNA by the use of ODN 2 incorporating S-nitroso thioguanosine. It has been demonstrated that the S-NO containing ODN 2 exhibits rapid and specific NO-transfer reaction to its complementary ODN 5 having dC or dmC at the target site. Thus, we have established an innovative method for the highly efficient and selective transfer of NO to cytidine and 5-methylcytidine.


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