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Nucleic Acids Symposium Series 2004 48(1):251-252; doi:10.1093/nass/48.1.251
© 2004 by Oxford University Press
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Synthesis of ferrocenyl carbodiimide as a novel ferrocenyl reagent of single stranded DNA

Kosuke Mukumoto, Takahiko Nojima and Shigeori Takenaka

Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan

Ferrocenyl carbodiimide (FcCDI) was newly synthesized as a ferrocenylation reagent for single stranded DNA. FcCDI could attach to oligodeoxyribonucleotides (ODN) through a covalent bond with thyminc and guanine bases. The reactivity of thymine with FcCDI was higher than that of guanine, but independent of their location on the DNA sequence. After a single stranded DNA was treated with FcCDI, resulting ODN could be detected electrochemically by a redox signal deriving from the ferrocenyl moiety with a DNA probe-immobilized electrode. These results suggest that the labeling of DNA with FcCDI can be applied to rapid analysis of electrochemical genosensors.


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