© 2004 by Oxford University Press
Conformational change of a nucleotide by a base-pair mimic nucleoside in the complementary DNA strand
1 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 2 Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan, 3 Kinki University School of Humanity-Oriented Science and Engineering, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan
On the basis of the non-covalent bond interactions in nucleic acids, we have synthesized a novel adenosine derivative tethering a phenyl group. Structure and thermal stability of the DNA duplexes bearing the adenosine derivative in the middle of a strand were investigated by CD spectra and the UV melting curves. We found that the conformation and the interactions of the duplexes were similar regardless of the nucleotide facing with the adenosine derivative in a duplex. This observation suggests the base pair-mimic geometry of the adenosine derivative accompanied with a conformational change of the nucleotide in the complementary strand.