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Nucleic Acids Symposium Series 2004 48(1):267-268; doi:10.1093/nass/48.1.267
© 2004 by Oxford University Press
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Nucleation-synchronized strand displacement for highly sensitive DNA analysis

Kazuya Hirata1, Yuichi Sato2, Arihiro Kano2, Toshihiro Akaike1 and Atsushi Maruyama2,3

1 Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori, Yokohama 226-8501, Japan, 2 PRESTO, Japan Science and Technology Agency, 3 Institute for Materials Chemistry and Engineering, Kyushu University

Nucleation process is a rate-limiting step of DNA strand exchange reactions between double helical DNA and its complementary DNA. Here we designed the double stranded probes that have single stranded portion. The single stranded portion is responsible for the nucleation process and mismatch recognition. A single base mismatch in 25mer DNAs could be detected in a few minutes at ambient temperatures. Using this method, various types of SNPs are rapidly and reliably detected without any aids of enzymes or a special instrument.


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