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Nucleic Acids Symposium Series 2004 48(1):70-71; doi:10.1093/nass/48.1.70
© 2004 by Oxford University Press
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Electrochemical behavior of gold electrodes modified with photosensitizer-tethered DNA

Akimitsu Okamoto1, Taku Kamei1,3, Kazuo Tanaka1,3 and Isao Saito2,3

1 Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan, 2 Faculty of Engineering, NEW CAT Institute, Nihon University, Koriyama 963-8642, Japan, 3 SORST, Japan Science and Technology Corporation

We investigated electrochemical characteristics of gold electrodes modified with photosensitizer-tethered DNA. We synthesized 3'-thiol-modified DNA strands (ODN-SH) and 3'-anthraquinone-modified DNA strands (AQ-ODN). We prepared an AQ-ODN/ODN-SH duplex-modified electrode through hybridization of AQ-ODN with ODN-SH on the gold electrode. We used an amperometric technique for its photocurrent measurement. The large photocurrent was observed by irradiation of a DNA modified electrode at 365 nm. We also measured photocurrent with different DNA sequences on gold electrodes.


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