Skip Navigation

Nucleic Acids Symposium Series 2004 48(1):77-78; doi:10.1093/nass/48.1.77
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ogino, M.
Right arrow Articles by Fujimoto, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ogino, M.
Right arrow Articles by Fujimoto, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Reversible DNA photoligation via {alpha}-5-cyanovinyldeoxyuridine

Masayuki Ogino1, Yoshinaga Yoshimura2, Akio Nakazawa3, Isao Saito3 and Kenzo Fujimoto1,2

1 The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan, 2 PRESTO, Japan Science and Technology Corporation, Japan, 3 Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan

An efficient and reversible Temmplate- directed Photoligation of oligodeoxynucleotides (ODNs) using {alpha}-5-cyanovinyldeoxyuridine ({alpha}-CNVU) was described. When ODN containing {alpha}-CNVU at the 3' end was photoirradiated with ODN containing thymidine at the 5' end in the presence of a template ODN, an efficient photoligation was observed. The ligated ODN can be split site- selectively to regenerate parent ODN by photoirradition at the 312 nm.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.