Skip Navigation

Nucleic Acids Symposium Series 2005 49(1):127-128; doi:10.1093/nass/49.1.127
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ohkubo, A.
Right arrow Articles by Sekine, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohkubo, A.
Right arrow Articles by Sekine, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2005 Oxford University Press

Development of new N-unprotected phosphoramidite building blocks having a silyl-type linker

Akihiro Ohkubo1,3, Katsufumi Aoki1,3, Haruhiko Taguchi1,3, Kohji Seio2,3 and Mitsuo Sekine1

1 Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8501, Japan, 2 Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8503, Japan, 3 CREST, JST

New silyl-type linkers 2 and 3 were developed for the synthesis of oligonucleotides having base-labile functional groups in the phosphoramidite method without base protection. These linkers could be introduced into polymer supports in high yields and easily cleaved under neutral conditions. Particularly, the use of the N-unprotected phosphoramidite building block 3 having a silyl-type linker enabled us to synthesize oligodeoxynucleotides without protecting groups for the amino groups of 3'-terminal deoxyribonucleosides.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.