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Nucleic Acids Symposium Series 2005 49(1):359-360; doi:10.1093/nass/49.1.359
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© 2005 Oxford University Press

SNPs (single nucleotide polymorphisms) detection based on the formation of cholic-acid binding DNA aptamer

Teru Kato1, Atsuko Yuki2, Kazuyoshi Yano1, Yoshiko Arikawa2 and Isao Karube1

1 School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan, 2 Graduate School of Science, Japan Women's University, 2-8-2 Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan

Simple and rapid methods for the detection of single nucleotide polymorphisms (SNPs) are required to analyze the genetic information. We have previously isolated DNA aptamers that bind to cholic acid by an in vitro selection method. A common feature of the sequences of the aptamers was that they included a secondary structure, a three-way junction, in which three stems were connected at the junction. It was also revealed that three-way junctions with fully matched three stems bind cholic acid whereas single-base substitutions at the junction lose binding affinity completely. Here we report a new method for the detection of single nucleotide mutations that is based on the formation of fully matched three-way junctions which bind to cholic acid.


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