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Nucleic Acids Symposium Series 2005 49(1):51-52; doi:10.1093/nass/49.1.51
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© 2005 Oxford University Press

NMR spectroscopic analyses of functional nucleic acids-metal interaction and their solution structure analyses

Yoshiyuki Tanaka1, Hiroshi Yamaguchi1, Shuji Oda1, Chojiro Kojima2, Akira Ono3, Kazunari Taira4,5 and Yoshinori Kondo1

1 Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan, 2 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan, 3 Department of Applied Chemistry, Faculty of Engineering, Kanagawa University, Japan, 4 Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan, 5 Gene Function Research Center, AIST, Japan

We have studied complexations of functional nucleic acids and metal ions, by means of NMR spectroscopy. In the case of hammerhead ribozymes, they have a metal ion-binding motif in their core sequences. Upon the metallation of N7 of a guanosine in an RNA duplex modelled after hammerhead ribozymes, 20 ppm higher field shift of N7 was observed in 1-dimensional (1D) 15N NMR spectra. It was found that metal ion binding to nucleobases were detectable with 15N NMR spectroscopy.


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