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Nucleic Acids Symposium Series 2005 49(1):67-68; doi:10.1093/nass/49.1.67
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© 2005 Oxford University Press

The development of crystallization and X-ray crystallography method of long stem DNA

Hirofumi Ohishi1, Koji Tukamoto2, Mamiko Odoko3, Kazimierz Grzeskowiak4, Naoyoshi Maezaki2, Nobuo Okabe3, Tetsuaki Tanaka2 and Toshimasa Ishida1

1 Osaka University of Pharmaceutical Sciences 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan, 2 Graduate School of Pharmaceutical Sciences Osaka University 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan, 3 School of Pharmaceutical Sciences Kinki University 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan, 4 University of California Los Angeles

We clarified stabilization mechanism of left-handed Z-DNA by X-ray crystal structure analysis. I was able to achieve it by burying big minor groove of Z-DNA because metal ion and polyamine connected it to minor groove of Z-DNA. On that occasion I understood that an electric charge and length of polyamine gave big influence to a combination brain very much. I succeeded in crystallization of Z-DNA of a long chain, X-ray crystal structure analysis by examining these combination ability.


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