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Nucleic Acids Symposium Series 2005 49(1):73-74; doi:10.1093/nass/49.1.73
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© 2005 Oxford University Press

Gene manipulation using artificial restriction DNA cutter

Yoji Yamamoto, Jing-Min Zhou, Takafumi Tomita, Akihiko Uehara, Yuichiro Aiba, Akira Watanabe, Hiroyuki Aburatani and Makoto Komiyama

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan

Artificial restriction DNA cutter (ARCUT), which we developed recently, was used for manipulation of plasmid DNA. PCR product was inserted into pBR322 plasmid vector using ARCUT, and E. coli cells were transfected with this recombinant plasmid DNA. Successful growth of cells shows that the recombination proceeds without any unexpected mutations. Furthermore, this artificial system was applied to PCR-free construction of chimera protein.


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