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Nucleic Acids Symposium Series 2005 49(1):91-92; doi:10.1093/nass/49.1.91
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© 2005 Oxford University Press

Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs)

Makoto Miyagishi1, Sahohime Matsumoto1, Hideo Akashi1, Hiroaki Kawasaki1, Taro Fukao1, Yoko Fukuda1, Masayuki Sano2, Yoshio Kato2, Yasuomi Takagi2, Yoshiyuki Tanaka2, Masaki Warashina2, Tomoko Kuwabara2, Shinya Y. Sawata2, Yutaka Ikeda2, Shun-ichi Kawahara2, Kaul C. Sunil2, Renu Wadhwa1,2 and Kazunari Taira1,2

1 Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan, 2 Gene Function Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan

Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.


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