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Nucleic Acids Symposium Series 2006 50(1):17-18; doi:10.1093/nass/nrl009
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© 2006 Oxford University Press

A ribonucleoside with pyrimido[4,5-d]pyrimidine-2,4,5,7-(1H,3H,6H,8H)-tetraone as a nucleobase, which universally binds to natural nucleosides

Kenji Kuroda1,2, Hidehiko Kodama2, Masanori Kataoka3 and Yoshihiro Hayakawa1,2

1 Graduate School of Information Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan, 2 CREST/JST, Nagoya University, Chikusa, Nagoya 464-8601, Japan, 3 Research Center for Computational Science, National Institutes for Natural Sciences, Okazaki 444-8787, Japan

A ribonucleoside with pyrimido[4,5-d]pyrimidine-2,4,5,7-(1H,3H,6H,8H)-tetraone, which is expected to serve as a universal base, was synthesized and binding affinity of this artificial nucleoside to natural nucleosides was investigated by UV analysis. As the result, it was revealed that the artificial derivative universally forms a base pair with four kinds of natural deoxyribonucleosides.


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