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Nucleic Acids Symposium Series 2006 50(1):255-256; doi:10.1093/nass/nrl127
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© 2006 Oxford University Press

Highly active artificial restriction enzyme composed of Ce(IV)/EDTA and PNA bearing phosphate group - Relationship between the promotion by phosphate and the structure of invasion complex -

Yuichiro Aiba, Yoji Yamamoto and Makoto Komiyama

Research center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan

Recently, we developed artificial restriction DNA cutter (ARCUT) composed of pseudo-complementary peptide nucleic acid (pcPNA) and Ce(IV)/EDTA complex (EDTA = ethylenediamine-N,N,N',N'-tetraacetate). Here we promoted the site-selective hydrolysis by attaching phosphate groups to the pcPNAs. The promotion by the phosphates increased with decreasing length of the gap-like site. Furthermore, the scission was successful even when phosphate groups were introduced to 0 base-gap system.


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