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Nucleic Acids Symposium Series 2006 50(1):257-258; doi:10.1093/nass/nrl128
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© 2006 Oxford University Press

Analysis of PPAR alpha function in human kidney cell line using siRNA

Keisuke Tachibana1, Naohiko Anzai2, Chihiro Ueda1, Tatsuya Katayama1, Takayoshi Kirino1, Rika Takahashi1, Daisuke Yamasaki1, Kenji Ishimoto1, Toshiya Tanaka3, Takao Hamakubo3, Yukihiko Ueda4, Hiroyuki Arai5, Juro Sakai3, Tatsuhiko Kodama3 and Takefumi Doi1

1 Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan, 2 Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan, 3 Laboratory for System Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan, 4 Research Institute, Shiga Medical Center, 5-4-30 Moriyama, Shiga 524-8524, Japan, 5 Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan

The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPAR{alpha} is mainly expressed in the liver, kidney, heart and muscle. PPAR{alpha} activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPAR{alpha} is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPAR{alpha} in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPAR{alpha} and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPAR{alpha} siRNA. These results suggest that PPAR{alpha} regulates fatty acid metabolism and body water homeostasis in this cell line.


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