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Nucleic Acids Symposium Series 2006 50(1):283-284; doi:10.1093/nass/nrl141
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© 2006 Oxford University Press

Visible sensing of nucleic acid sequences using a genetically encodable unmodified mRNA probe

Atsushi Narita, Kazumasa Ogawa, Shinsuke Sando and Yasuhiro Aoyama

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

We previously reported a molecular beacon-mRNA (MB-mRNA) strategy for nucleic acid detection/sensing in a cell-free translation system using unmodified RNA as a probe. Here in this presentation, we report that a combination with RNase H activity, which induces an additional process of irreversible cleavage of MB-domain, achieves an improved sequence selectivity (one nucleotide selectivity) and an enhanced sensitivity. This improved system finally enabled visible sensing of target nucleic acid sequence at a single nucleotide resolution under isothermal conditions.


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