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Nucleic Acids Symposium Series 2006 50(1):303-304; doi:10.1093/nass/nrl151
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© 2006 Oxford University Press

Estimation of DNA polymerase for improvement of rolling circle amplification

Takeo Yoshimura1, Saori Arikado2 and Shokichi Ohuchi1,2

1 Department of Biological Functions and Engineering, Kyushu Institute of Technology, Wakamatsu, Kitakyushu, Fukuoka 808-0196, Japan, 2 Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan

Rolling Circle Amplification (RCA) is a technique to amplify the oligoncleotides containing tandemly repetitive sequence motif. Various repetitive sequences of which the motif unit is the circular template can be synthesized by extending the primer. Additionally, RCA need not change the temperature regulatory processes like PCR, but RCA has the gene amplification efficiency similar to PCR. Although, it contains the problem of the sequence confirmation and the activity of polymerase as the DNA polymerase I. We comparatively estimated of the various polymerases for the improvement of the RCA. Five DNA polymerases were estimated for RCA.


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