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Nucleic Acids Symposium Series 2007 51(1):237-238; doi:10.1093/nass/nrm119
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© 2007 Oxford University Press

Effect of local structural disruption on the photochemical ligation between anthracene-modified DNA conjugates

Pelin Arslan1, Toshihiro Ihara1,2,*, Motoko Mukae1 and Akinori Jyo1

1Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan and
2PRESTO, Japan Science and Technology Agency, San-bancho Building 3-5 Sanbancho, Chiyodaku, Tokyo 332-0012, Japan

*Corresponding Author. toshi{at}chem.kumamoto-u.ac.jp

Abstract

DNA templated ligation reactions has attracted great interest in the field of DNA probing and medical diagnosis. To date some research groups reported a variety of chemical ligations, in which two DNAs with reactive groups that bound adjacently on the complementary strand were connected covalently to form a corresponding longer product. Here we introduced the photoligation of anthracene modified ODN conjugates through anthracene cyclodimer formation. The effect of the position of single base mismatch on the template was evaluated using six sequences with one-base displacements. The yield of the ligation was affected by mispairing in position-dependent manner. It would be due to the disruption of the local structure around the two anthracenes of both reaction partners.


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