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Nucleic Acids Symposium Series 2007 51(1):281-282; doi:10.1093/nass/nrm141
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© 2007 Oxford University Press

Sensing of nucleic acid sequences using unmodifiednucleic acid as a probe

Atsushi Narita, Shinsuke Sando* and Yasuhiro Aoyama

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

*Corresponding Author. ssando{at}sbchem.kyoto-u.ac.jp (S.S) or aoyamay{at}sbchem.kyoto-u.ac.jp

Abstract

We present a strategy to generate a light-up fluorophore-aptamer pair. The strategy was based on a modification of a conventional DNA-staining dye to suppress its affinity to the original targets, and subsequent re-selection of aptamers that would bind to the modified dye. Along this line, we prepared an environmental polarity-sensitive Hoechst derivative with low affinity to the usual AT-rich dsDNA targets. DNA aptamers, in vitro selected from a random pool, worked as a trigger to enhance the fluorescence of an otherwise nonfluorescent Hoechst derivative.


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