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Nucleic Acids Symposium Series 2007 51(1):29-30; doi:10.1093/nass/nrm015
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© 2007 Oxford University Press

Novel Applications of Locked Nucleic Acids

Rakesh N. Veedu, Birte Vester and Jesper Wengel*

Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK – 5230 Odense M, Denmark

*Corresponding Author. E-mail: jwe{at}ifk.sdu.dk

Abstract

Locked Nucleic Acid (LNA) nucleoside triphosphates were prepared and their substrate properties for different polymerases during primer extension and PCR experiments investigated. PhusionTM High Fidelity DNA polymerase and 9°NmTM DNA polymerase readily accept LNA nucleoside 5'-triphosphates as substrates in primer extension assays. However, in PCR assays, However, in PCR assays, DNA 9oNmTM polymerase proved to be the best for amplification employing the LNA-A nucleotide.


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