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Nucleic Acids Symposium Series 2007 51(1):335-336; doi:10.1093/nass/nrm168
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© 2007 Oxford University Press

SAXS measurement of aggregate of DNA modified gold nanoparticles

Shizuto Yamakoshi1,*, Yasuhiro Sakai1, Yuya Shinohara1, Yoshiyuki Amemiya1, Naoki Kanayama2, Tohru Takarada2, Mizuo Maeda2 and Kohzo Ito1

1Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8561, Japan and
2RIKEN,2-1 Hirosawa, Wako, Saitama 351-0198, Japan

*Corresponding Author. sizuto{at}molle.k.u-tokyo.ac.jp

Abstract

DNA-modified gold nanoparticles exhibit a unique aggregation behaviour that they form aggregation with fully complementary DNA but do not with the terminal-mismatched DNA at certain concentration of added salts. We studied the aggregation structure of the nanoparticles by small angle X-ray scattering (SAXS). The results indicated that the gap between surfaces of gold nanoparticles is nearly two times as long as DNA in B-form. This suggests that the ends of completely double-stranded DNA (dsDNA) conjugated on gold nanoparticles contact or slightly interdigitate with each other in the aggregation structure.


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