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Nucleic Acids Symposium Series 2007 51(1):339-340; doi:10.1093/nass/nrm170
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© 2007 Oxford University Press

Novel analysis for single nucleotide polymorphism using cationic comb-type copolymers

Naohiko Shimada1, Kentaro Muraki1, Taro Anai1, Arihiro Kano1 and Atsushi Maruyama1,2,*

1Institute for Materials Chemistry and Engineering, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-8581, Japan and
2CREST, Japan Science and Technology Agency, Saitasma, Japan

*Corresponding Author. maruyama{at}ms.ifoc.kyushu-u.ac.jp

Abstract

We have focused on the DNA strand exchange reaction (SER) as a format of a single-base mismatch detection. We employed a partially double-stranded (PDS) DNA probe having a single-stranded (ss) portion. We could rapidly recognize a single-base mismatch by the SER rates between the PDS probes and target ss DNAs. In the presence of cationic comb-type copolymers (PLL-g-Dex) and betaine, the PDS probes could clearly discriminate between A:T matched and A:G mismatched targets. Furthermore, the PDS probes could discriminate among homo- and hetero-type alleles.


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