Skip Navigation

Nucleic Acids Symposium Series 2007 51(1):353-354; doi:10.1093/nass/nrm177
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kondo, Y.
Right arrow Articles by Ito, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kondo, Y.
Right arrow Articles by Ito, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 Oxford University Press

Multiple chemical ligation under thermal cycle

Yuko Kondo1,2,*, Hiroshi Abe1, Hiroshi Jinmei1, Naoko Abe1, Kyoko Aikawa2, Isamu Matsumoto2 and Yoshihiro Ito1

1Nanomedical Engineering Laboratory, Discovery Research Institure, Riken, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan and
2Department of Chemistry & Biochemistry, Ochanomizu University, 2-1-1 Otsuka Bunkyo-ku, Tokyo 112-0012, Japan

*Yuko Kondo. yukok{at}riken.jp

Abstract

Enzymatic ligation methods are useful in diagnostic detection of DNA sequence. Here we describe the investigation of nonezymatic phosphorothioate-iodoacetyl DNA chemical ligation as a method for detection and identification of RNA and DNA. Specificity of ligation on DNA target is shown to yield discrimination of single point mutation as a drop in two magnitude. Although enzymatic liagtion shows very low activity for RNA target, this reaction is found to be very efficient on RNA target. This chemical ligation with RNA target completes 70% within a few seconds, which equal or overcome ligase enzyme-mediated ligation with DNA target. The reaction is also shown to exhibit a siginificant level of signal amplification under thermal cycle for short time. Further, we found recently that ligtion fidelity changed in function of chemical reactivity of probe. This trend was systematically investigated.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.