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Nucleic Acids Symposium Series 2007 51(1):357-358; doi:10.1093/nass/nrm179
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© 2007 Oxford University Press

Enzymatic Incorporation of 2-Amino-6-Vinylpurine Derivative

Shuhei Imoto1, Md. Monsur Ali2, Yingfu Li2, Shigeki Sasaki3,4 and Fumi Nagatsugi1,4,*

1Institute of Multidisciplinary Research for Advanced Materials Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-city, Miyagi, JAPAN, 2Dept of Biochemistry and Biomedical Sciences, McMaster University, 1200, Main Street West, Hamilton, ON L8N 3Z5, CANADA, 3Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Japan, 4CREST, Japan Science and Technology Agency

*Corresponding Author; Fumi Nagatsugi, nagatugi{at}tagen.tohoku.ac.jp

Abstract

Modi fied nucleotides are incorporated by enzymatic reactions into a DNA oligonulce otide to provide additional functionalities, which has shown pote ntial applications in a wi de variety of fiel ds. A l arge number of modified nucleoti de tri phos phates have been incor porated to DNA or RNA by pol ymerases. We have previously describe d that the sulfide deri vati ves (1) of 2-amino-6-vinyl purine nucle oside (2, AVP) are acti vated wi thin duplex to form cross-link toward cyti dine selectively at the target site. In this paper, we describe the preparati on of the tri phos phate deri vative of 2'-deoxyribosyl-2-amino-6-(2-methyl thioe thyl) purine nucleoside (5, dAVP(S Me)-TP), and its enzymatic incor poration to DNA.


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