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Nucleic Acids Symposium Series 2007 51(1):453-454; doi:10.1093/nass/nrm227
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© 2007 Oxford University Press

Monitoring of Eco RI-catalyzed cleavage reaction of fluorescent-labeled heterochiral DNA

Hidehito Urata*, Chihiro Tamaki, Miki Matsuno, Shun-ichi Wada and Masao Akagi

Osaka university of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan

*Corresponding author. urata{at}gly.oups.ac.jp

Abstract

We have found the unusual reactivity of a heterochiral oligodeoxynucleotide toward restriction endonuclease Eco RI. To conduct the kinetic analysis of the reaction, fluorescent-labeled single-stranded oligodeoxynucleotide molecular beacons were designed and synthesized. The beacons showed a remarkable fluorescence response by addition of Eco RI. The results promise that the beacon could be an effective tool for the kinetic analysis of Eco RI-catalyzed cleavage reaction of the heterochiral oligodeoxynucleotide.


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