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Nucleic Acids Symposium Series 2008 52(1):353-354; doi:10.1093/nass/nrn178
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

Fluorogenic probe triggered by reduction for nucleic acids sensing

Kazuhiro Furukawa1,2, Hiroshi Abe1,*, Jin Wang1, Kazuma Oki1,2, Miwako Uda1,2, Satoshi Tsuneda2 and Yoshihiro Ito1,*

1Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 Japan, 2Department of Life Science and Medical Bio-Science, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan

*Corresponding Author. E-mail: h-abe{at}riken.jp (H. A.) or y-ito{at}riken.jp (Y. I.)

Abstract

A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10–20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.


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