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Nucleic Acids Symposium Series 2008 52(1):487-488; doi:10.1093/nass/nrn247
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

RNA aptamers specifically interact with the Fc region of mouse immunoglobulin G

Nobuya Sakai1,2, Hiromi Masuda1, Joe Akitomi1, Hirotaka Yagi1, Yoshihito Yoshida1, Katsunori Horii1, Makio Furuichi1 and Iwao Waga1,*

1VALWAY Technology Center, NEC Soft, Ltd., Tokyo, 136-8627, Japan
2Institute of Applied Beam Science, Graduate School of Science and Engineering, Ibaraki University, Nakanarusawa, Hitachi 316-8511, Japan

*Corresponding Author. Iwao Waga, E-mail: wagaiwao{at}mxc.nes.nec.co.jp

Abstract

We have designed the in vitro selection method to obtain some aptamers such as a general antibody-probing agent, which might bind to the constant regions of mouse immunoglobulin G (IgG) subclasses. As a consequence, one of the selected aptamers found to recognize mouse IgG1, 2a, and 3 subclasses. According to the binding assay, it is suggested that this aptamer recognizes the constant regions of mouse IgG subclass. In addition, this aptamer could recognize the only native form of mouse IgGs but the denatured IgGs. These features show the advantage of the aptamer as an antibody-probing agent rather than the usual secondary antibodies.


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