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Nucleic Acids Symposium Series 2008 52(1):505-506; doi:10.1093/nass/nrn256
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

Nanocircular RNAs for RNA interference

Hiroshi Abe1, Naoko Abe1, Mitsuru Harada1,2, Satoshi Tsuneda2 and Yoshihiro Ito1

1Nano Medical Engineering Laboratory, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako-Shi, Saitama, 351-0198 Japan and 2Department of Life Science and Medical Bio-Science, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan

*Corresponding Author. E-mail: h-abe{at}riken.jp

Abstract

We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops1. RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-strandedRNA in cells, although this RNA is resistant todegradation in serum. The structure was optimized tomaximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.


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