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Nucleic Acids Symposium Series 2008 52(1):513-514; doi:10.1093/nass/nrn260
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

In vitro selection of RNA aptamer to hemin

Mingzhe Liu, Takuma Kagahara, Hiroshi Abe and Yoshihiro Ito*

Nano Medical Engineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, JAPAN

*Corresponding Author. E-mail: y-ito{at}riken.jp

Abstract

A new RNA aptamer-binding hemin was synthesized by the in vitro selection (SELEX) method. A pool of 103 bases single strand DNAs containing a randomized sequence of 59 bases was synthesized. The pool was incubated with hemin on hemin-immobilized affinity column. Bound RNAs were eluted off with hemin solution and amplified by PCR. After 3 rounds of selection process, the selected RNAs were cloned and sequenced. Some RNA aptamers, which have affinity to hemin, was selected.


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