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Nucleic Acids Symposium Series 2008 52(1):53-54; doi:10.1093/nass/nrn027
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

Novel base-functionalized DNA. Efficient methodology for construction and bioanalytical applications

Michal Hocek1,*, Milan Vrábel1, Hana Cahová1, Jan Riedl1, Lubica Kalachová1, Hana Pivonková2, Petra Horáková2, Ludek Havran2 and Miroslav Fojta2

1 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, CZ-16610 Prague 6, Czech Republic;
2 Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-61265 Brno, Czech Republic

E-mail: hocek{at}uochb.cas.cz

Abstract

A novel efficient two-step methodology for the construction of base-functionalized DNA is based on direct aqueous cross-coupling reactions of unprotected nucleoside triphosphates followed by polymerase incorporation. Preliminary applications of the modified DNA in electrochemical detection and bioanalysis are outlined.


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