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Nucleic Acids Symposium Series 2008 52(1):59-60; doi:10.1093/nass/nrn030
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing

Mikiko C. Siomi1,2,* and Haruhiko Siomi1

1Keio University School of Medicine, Tokyo 160-8582, Japan and 2JST, CREST, Saitama 332-0012, Japan

*Corresponding author. E-mail: siomim{at}sc.itc.keio.ac.jp

Abstract

Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave in respect to small RNA association and target RNA destruction has remained unclear. Recently, we produced monoclonal antibodies for individual hAgos and characterized small RNAs specifically associated with hAgo2 and hAgo3 endogenously expressed in Jurkat cells.


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