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Nucleic Acids Symposium Series 2008 52(1):73-74; doi:10.1093/nass/nrn037
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© 2008 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: Joint Symposium of the 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and the 35th International Symposium on Nucleic Acids Chemistry [View the issue table of contents]

In vitro selection of a DNAzyme with three modified nucleotides

Marcel Hollenstein, Christopher Hipolito, Curtis Lam and David Perrin*

Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver BC, V6T 1Z1, Canada

*Corresponding Author. E-mail: dperrin{at}chem.ubc.ca

Abstract

Three modified nucleosides have been used in the in vitro selection of the self-cleaving DNAzyme 9-33. This DNAzyme operates in the absence of divalent metal cations with a first-order constant of {approx} 0.05 min–1.


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