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Nucleic Acids Symposium Series 2009 53(1):17-18; doi:10.1093/nass/nrp009
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© 2009 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: The 6th International Symposium on Nucleic Acids Chemistry (36th Symposium on Nucleic Acids Chemistry) [View the issue table of contents]

Metal ion binding of modified pyrimidine-base pairs in DNA duplexes

Akira Ono, Kenji Iwamoto, Kumiko Sugiyama and Itaru Okamoto

Department of Material & Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686, Japan

*Corresponding author. E-mail: akiraono{at}kanagawa-u.ac.jp

Abstract

We report the synthesis and metal ion-binding properties of DNA duplexes containing 5-substituted uracil (XU) pairs, such as 5-bromouracil, 5-fluorouracil and 5-cyanouracil pairs. Thermal denaturation studies of these modified DNA duplexes revealed that the DNA duplexes were stabilized in the presence of HgII ions in acidic and neutral solutions. On the other hand, the duplexes were stabilized in the presence of HgII and AgI ions. These results indicated that XU-HgII-XU complex was formed in the acidic and neutral solutions, then, in the basic solutions XU-AgI-XU complex as well as the XU-HgII-XU complex were formed. ESI-TOF MS analysis also indicated formation of the metal ion-DNA complexes.


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