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Nucleic Acids Symposium Series 2009 53(1):21-22; doi:10.1093/nass/nrp011
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© 2009 Oxford University Press

This article appears in the following Nucleic Acid Symposium Series issue: The 6th International Symposium on Nucleic Acids Chemistry (36th Symposium on Nucleic Acids Chemistry) [View the issue table of contents]

Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine and 5-methyl-2-thiodeoxycytosine

Akihiro Ohkubo, Kenichi Miyata, Hirosuke Tsunoda, Kohji Seio and Mitsuo Sekine

Department of Life Science, Graduate School of Bioscience and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan

*Corresponding author. E-mail: msekine{at}bio.titech.ac.jp

Abstract

For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides (TFO) containing 8-thioxodeoxyadenine (s8A) residues in place of the protonated cytosines (Cs) required for the third base pairing with DNA duplexes. Consequently, it was found that s8A exhibited much stronger hybridization ability than C under neutral conditions when four s8A bases were arranged in a consecutive sequence. Moreover, we also synthesized TFOs containing 5-methyl-2-thiocytosines and examined their ability of triplex formation.


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